Journal of Pharmacognosy and Phytochemistry
Vol. 2, Issue 5 (2014)
Single dose oral toxicity study of nano formulated extract of Picrorhiza kurroa in wistar rats
M. Hemanth kumar, C .Ramesh
Single dose oral toxicity study of Nano formulation made from extract of Picrorhiza kurroa was carried out in Wistar Rats. The objective of this study was to assess the single dose oral toxicity of nano formulation of Picrorhiza Kurroa extract in Wistar Rats, at a dosage of 200, 1000 or 2000 mg/kg body weight. Forty Wistar rats were distributed to 4 dose groups, consisting of 10 rats (5 male + 5 female) each. Three groups were orally administered with Picrorhiza Kurroa extract at doses of 200 mg/kg, 1000 mg/kg or 2000 mg/kg body weight and observed daily for 14 days. Animals were sacrificed on Day 15. The control (0 mg/kg) group received the vehicle (0.5 % carboxy methylcellulose). The dose volume was given at the rate of 20 ml/kg body weight. All the rats were examined daily for signs of toxicity and mortality, and were subjected to detailed clinical examination before initiation of the study and weekly thereafter during the exposure period. Body weights and food consumption were recorded twice weekly. All animals were sacrificed at termination on day 15 and were subjected to detailed gross pathology. In the present study, nano formulation of Picrorhiza Kurroa extract has not caused any mortality or any symptoms of toxicity at and up to a dose of 2000 mg/kg body weight (Limit Dose), under the conditions of the study.
Pages: 138-145 | 1350 Views 27 Downloads
M. Hemanth kumar, C .Ramesh. Single dose oral toxicity study of nano formulated extract of Picrorhiza kurroa in wistar rats. J Pharmacogn Phytochem 2014;2(5):138-145.