• Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal
Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 2, Issue 6 (2014)

High frequency plant regeneration from shoot tip explants of Citrullus colocynthis (Linn.) Schrad. –An important medicinal herb

Author(s):

Mahesh Chand Meena, Rishi Kesh Meena and Vidya Patni

Abstract:
A high frequency and rapid regeneration protocol was developed from shoot tip explants of Citrullus colocynthis on MS medium supplemented with BAP (0.5 mg/l) and NAA (0.5 mg/l). Highest number of shoots (23.0 ± 0.567) was obtained on MS medium containing BAP (0.5 mg/l) and NAA (0.5 mg/l). The regenerated shoots were further elongated on same medium. In vitro shoots were excised from shoot clumps and transferred to rooting medium containing IBA (4.0 mg/l) with 0.2% activated charcoal. The rooted plants were hardened in polycups containing sterile soil and vermiculate and finally well established in the field; survival rate was 60%. This is the first report of direct in vitro plantlet regeneration in Citrullus colocynthis from shoot tip explant.

Pages: 53-56  |  1592 Views  40 Downloads

How to cite this article:
Mahesh Chand Meena, Rishi Kesh Meena and Vidya Patni. High frequency plant regeneration from shoot tip explants of Citrullus colocynthis (Linn.) Schrad. –An important medicinal herb. J Pharmacogn Phytochem 2014;2(6):53-56.