Journal of Pharmacognosy and Phytochemistry
Vol. 3, Issue 4 (2014)
Phytochemical analysis and antibacterial activity of Maytenus emarginata leaf and stem
Pooja Moteriya, Hemali Padalia, Tejas Rathod, Disha Menpara and Sumitra Chanda
Traditional medicine is the main source of medical care for a great percentage of the population of the developing world. Medicinal plants have been a valuable source of natural active phytoconsituents that play an important role in treatment of many human diseases. In the present study, leaf and stem of Maytenus emarginata was evaluated for its antibacterial potential and polyphenol content. The extraction was done by individual cold percolation method using six different solvents viz., hexane, toluene, ethyl acetate, acetone, methanol and water. Phenolic and flavonoid content of different extracts was determined using Folin-ciocalteu assays and aluminium chloride colorimetric method respectively. Qualitative phytochemical analysis of the crude powder of M. emarginata leaf and stem was carried out for various phytoconstituents. The antimicrobial activity was evaluated by an agar well diffusion method against eight different pathogenic Gram negative and Gram positive bacteria. The extractive yield was maximum in aqueous and methanol extracts both in leaf and stem. Total phenol content was maximum in polar solvents methanol and acetone extracts both in leaf and stem. The solvent extracts of stem showed better antibacterial activity than a leaf. This may be because of the difference in the presence of phytoconsituents present in them.
Pages: 202-208 | 1226 Views 31 Downloads
Pooja Moteriya, Hemali Padalia, Tejas Rathod, Disha Menpara and Sumitra Chanda. Phytochemical analysis and antibacterial activity of Maytenus emarginata leaf and stem. J Pharmacogn Phytochem 2014;3(4):202-208.