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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 3, Issue 6 (2015)

Comparative extraction methods, phytochemical constituents, fluorescence analysis and HPLC validation of rosmarinic acid content in Mentha piperita, Mentha longifolia and Osimum basilicum

Author(s):

Aveen Nozad Adham

Abstract:
The present investigation is aimed to compare the percentage value of Mentha Piperita, Mentha longifolia and Osimum basilicum extracts by using different solvent and extraction methods, phytochemical screening of constituents present in dry leaves, also to study fluorescent characteristics of leaves powder which are responsible for the medicinal properties of the plant using various standard tests and HPLC analysis of rosmarinic acid content in three labiatae species. The highest percentage extract was yielded from Mentha longifolia (18.5%) by using maceration methods and ethanol 80% solvent. Phytochemical analysis revealed the presence of alkaloids, saponins, phenols, flavonoids, terpenoids, cardioactive glycosides, tannins, carbohydrates in dry leaf extracts. Presence of alkaloids and phenolic acid was determined in high quantity in Mentha longifolia, while saponins present in high quantity in Mentha Piperita and flavonoids in Osimum basilicum. The fluorescent characteristics of leaves powder with various chemical reagents were noted under visible and UV light. HPLC analysis of dry leaf extracts revealed presence of rosmarinic acid among the investigated plants and in higher percentage in Osimum basilicum (0.306%).

Pages: 130-139  |  4747 Views  1579 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Aveen Nozad Adham. Comparative extraction methods, phytochemical constituents, fluorescence analysis and HPLC validation of rosmarinic acid content in Mentha piperita, Mentha longifolia and Osimum basilicum. J Pharmacogn Phytochem 2015;3(6):130-139.

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