Journal of Pharmacognosy and Phytochemistry
Vol. 3, Issue 6 (2015)
Phytochemical studies, total phenolic and flavonoids content and evaluation of antiradical activity of the extracts of the leaves from Dischistocalyx sp. (AcanthacÃ©es)
TimolÃ©on Andzi-BarhÃ©, Klo Kevin Massala, Louis ClÃ©ment Obame Engonga, Jacques Lebibi
The present study appears in the setting of the valorization of food and medicinal plants of Gabon. The traditional use of Dischistocalyx sp for the treatment of anemia and the absence of scientific works on this species incited us to achieve our survey on this plant. The phytochemical screening, The Folin-Ciocalteu method, the method of aluminum trichlorure (AlCl3, 2%) and DPPH radical was used, respectively, to identify the chemical groups content of Dischistocalyx sp, to quantify flavonoids and polyphenols content and to measure the Antiradical activity. Four extracts (water extract, ethanolic-water extract, ethanolic-acidified extract and ethanolic extract) have been analyzed. The qualitative analysis permitted to identify the presence of polyphenols, anthocyanins, tannins, triterpenoids, reducing sugars, flavonoids, glycosides and saponins in the different extracts. Quantitative analysis showed that the content in flavonoids was raised more in ethanolic-acid extract (330.406 Â± 0.021 mg EQ/g) and total polyphenols in the ethanolic-water extract (290.33 Â± 0.037 mg EAG/g). Radical Scavenging Activity (%RSA) increasing in the following order: % RSA (water extract) < % RSA (ethanolic extract) < % RSA (ethanolic-acidified extract) <% RSA (ethanolic-water extract).
Pages: 174-178 | 1952 Views 248 Downloads
TimolÃ©on Andzi-BarhÃ©, Klo Kevin Massala, Louis ClÃ©ment Obame Engonga, Jacques Lebibi. Phytochemical studies, total phenolic and flavonoids content and evaluation of antiradical activity of the extracts of the leaves from Dischistocalyx sp. (AcanthacÃ©es). J Pharmacogn Phytochem 2015;3(6):174-178.