Journal of Pharmacognosy and Phytochemistry
Vol. 3, Issue 6 (2015)
Paralysis and death of Pheretima posthuma and fungal growth inhibition by leaf extracts of Clerodendrum infortunatum L.
Mir Haris, Riaz Mahmood*, Haseebur Rahman, Nazneen, Venkatesh
In the modern world, the interest in the traditional cures is growing rapidly because of more side effects and raising prices in synthetic drugs. In this outlook herbal medicines play a great role to substitute the synthetic drugs with uncomplicated accessibility through the world. The aim of this study was to evaluate and authenticate the anthelmintic and antifungal efficacy of C. infortunatum leaf extracts in adult earthworms (Pheretima posthuma) and different fungal strains. The effectiveness of the plant extracts was assessed by monitoring the paralysis and death time of experimental earthworms, as well as the zone of inhibition in different fungal strains. The earth worms were treated with hexane, chloroform, ethyl acetate and ethanol extracts at a concentration of 25, 50 and 100 mg/ml of DMSO. Among these extracts ethanol and ethyl acetate have shown less time of paralysis (16 and 18.67 min) and death (46 and 48 min) at a concentration of 100 mg/mL compared to standard Piperazine citrate. The efficiency of hexane and chloroform extracts was recorded capable of paralysis and death of earthworms comparatively low to ethanol and ethyl acetate extracts. The same extracts were also subjected to antifungal screening against different fungal strains of Aspergillus niger, Aspergillus fumigates, Aspergillus flavorus and Candida albicans using agar well diffusion method at a concentration of 50, 75, 100 mg/mL and the zone of inhibition in millimeter (mm) has been recorded compared to reference standard Amphotericin (1 mg/mL). Among these extracts most have been proved effective against Aspergillus fumigates. The chloroform exhibited highest activity against Aspergillus fumigates (10.83 mm) at a concentration of 75mg/mL while as ethanol extract (100mg/mL) has shown intermediate inhibition (3.17 mm) to Aspergillus flavorus. None of the extracts have proven to be effective against Aspergillus niger and Candida albicans. The experimental proof of the current study proposes that leaves of C. infortunatum hold specific phytoconstituents which promote their use against the anthelminthes and fungal infections.
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Mir Haris, Riaz Mahmood*, Haseebur Rahman, Nazneen, Venkatesh. Paralysis and death of Pheretima posthuma and fungal growth inhibition by leaf extracts of Clerodendrum infortunatum L.. J Pharmacogn Phytochem 2015;3(6):188-192.