Journal of Pharmacognosy and Phytochemistry
Vol. 4, Issue 1 (2015)
HPTLC and HPLC analysis of T. chebula extracts prepared using microwave and ultrasonication assisted extraction methods
Rency Elizabeth Thomas, S. D. Kamat, D. V. Kamat
The medicinal value of the plants lies in the bioactive phytochemical constituents that produce definite physiological effects on human body. These natural compounds form the base of modern drugs. Thus, it is very important to develop effective and selective extraction methods for the recovery of these compounds. The aim of this study is to prepare Terminalia chebula extracts were using the microwave and ultrasonication assisted extraction methods and to determine the efficiency of the extraction method by a comparative study Methods:
Terminalia chebula extracts were prepared using methods of MAE and UAE. The extracts were analysed by HPLC. The tannin and gallic acid content of the extracts were determined and compared with that of the conventional extract. Fingerprint profiling of the extracts using HPTLC was also done. Results:
The study revealed an increase in the concentration of tannin and gallic acid establishing the superiority of the microwave and sonication extractions compared to the conventional extraction method. The HPTLC profile of the conventional, microwave and sonication extracts of T. chebula was compared and the difference in the peak display of the extracts is indicative of additional compounds being extracted as well as an increase in the concentration of certain compounds Conclusion:
Thus it can be concluded that microwave and ultrasonication assisted extraction methods can be useful tool for phytochemical extraction.
Pages: 192-196 | 2046 Views 499 Downloads
Rency Elizabeth Thomas, S. D. Kamat, D. V. Kamat. HPTLC and HPLC analysis of T. chebula extracts prepared using microwave and ultrasonication assisted extraction methods. J Pharmacogn Phytochem 2015;4(1):192-196.