Author(s):

Pritesh Ranjan Dash, Md Sohel Rana, Mohammad Shawkat Ali

Abstract: The present study was aimed to evaluate the possible analgesic and cytotoxic activities of the ethanol extract of Commelina appendiculata (EECA) (Family: Commelinaceae). Acetic acid-induced writhing, hot plate and tail immersion methods in swiss albino mice at the doses of 100 and 200 mg/kg body weight, p.o were used for the determination of analgesic activity. Brine shrimp lethality bioassay was carried out for assessing the cytotoxicity. Phytochemical screening of ethanol extract of C. appendiculata demonstrated the presence of carbohydrates, flavonoids, tannins, glycosides and alkaloids. In analgesic activity study, both doses of the extract produced a significant (p < 0.05 and p <0.001) analgesic action in a dose dependent manner. In acetic acid induced writhing method, EECA (200 mg/kg) showed the most significant analgesic activity with writhing inhibition of 76.27% where the standard drug Diclofenac-Na (25mg/kg) and Aspirin (100 mg/kg) showed 80.72% and 61.94% inhibition. In hot plate and tail immersion tests, EECA (200 mg/kg) produced maximum 52.56% and 76.58% nociception inhibition of thermal stimulus respectively. In this study, Morphine (5mg/kg, i.p.) was used as standard. With the EECA, significant lethality was found with LC50 value of 26.3 μg/ml while the LC50 of vincristine sulphate was 0.52 μg/ml. The results from the present studies reveal the analgesic and cytotoxic properties of the ethanol extract of C. appendiculata. So, the plant may be further investigated to find out for its pharmacological active natural products

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