Journal of Pharmacognosy and Phytochemistry
Vol. 4, Issue 4 (2015)
Anti-Proliferative and Cytotoxic Effects of Azima tetracantha Lam. On Cervical Cancer Cell Line (HeLa) and Human Peripheral Lymphocyte (HPL)
Gayathri Gopalakrishnan, Bindu Rajeshwary Nair, Babu V
Lam. (Family: Salvadoraceae, Common name: Needle bush) is distributed along the coastal areas of India. It is a spiny, evergreen shrub with a tendency to scramble. In the present study, the anti-cancer as well as cytotoxic properties of methanol leaf extract of Azima tetracantha
was determined, in vitro
, against the cancer cell line HeLa and Human peripheral Lymphocyte (HPL) respectively. MTT assay was used to determine cell viability and LDH leakage assay was used to determine the cell permeability in both HeLa and HPL cells. A dose dependant cell proliferation inhibitory activity was observed for the methanol extract (IC50= <100Âµg/ml) against HeLa. However, the extract remained less toxic to normal cells, HPL. Thus, the present study revealed that the methanolic extract of Azima tetracantha
possess chemoprotective effect and can be used as a potent agent in treating cancer.
Pages: 161-164 | 1465 Views 51 Downloads
Gayathri Gopalakrishnan, Bindu Rajeshwary Nair, Babu V. Anti-Proliferative and Cytotoxic Effects of Azima tetracantha Lam. On Cervical Cancer Cell Line (HeLa) and Human Peripheral Lymphocyte (HPL). J Pharmacogn Phytochem 2015;4(4):161-164.