Journal of Pharmacognosy and Phytochemistry
Vol. 4, Issue 4 (2015)
Chemical composition and antibacterial activity of extracts and compounds isolated from Citrullus colocynthis (L.) Schrad
Rachid Chawech, Dhekra Mhalla, Mohamed Trigui, Mohamed Mihoubi, Nicolas Fabre, Raoudha Jarraya
The aim of this study was the phytochemical screening of different extracts of leaves and stems prepared with three increasing polarity solvents (n-hexane, ethyl acetate and methanol) of Citrullus colocynthis.
Our main goal was to investigate the antibacterial activity of different extracts and compounds isolated from ethyl acetate extract of leaves using the agar disc well-diffusion method. rnFounded results showed that ethyl acetate extracts of leaves and stems possess potential antibacterial activity compared to other extracts against tested Gram-positive and negative bacterial strains. A bioguided isolation of the ethyl acetate extract obtainded from the leaves led to the identification of Cucurbitacin E and the Gluco-Cucurbitacine E. Moreover, a LC-MS analysis of ethyl acetate leaf extract of C. colocynthis
permitted the identification of eight other cucurbitacins. Antibacterial activities of the isolated compounds emphasize the importance of sugar moiety. The most important minimum inhibitory concentrations (MIC) values were obtained for the ethyl acetate extract 0.625 mg/mL against Bacillus cereus
E 1.25 mg/mL against both Bacillus cereus
and Enterococcus faecalis.
Pages: 197-203 | 2446 Views 394 Downloads
Rachid Chawech, Dhekra Mhalla, Mohamed Trigui, Mohamed Mihoubi, Nicolas Fabre, Raoudha Jarraya. Chemical composition and antibacterial activity of extracts and compounds isolated from Citrullus colocynthis (L.) Schrad. J Pharmacogn Phytochem 2015;4(4):197-203.