Journal of Pharmacognosy and Phytochemistry
Vol. 4, Issue 4 (2015)
Evaluation of chemical composition, cytostatic and anti-proliferative effects of ethanolic roots extract of Securidaca longepedunculata (Fresen)
Ogukwe CE, Okhale SE, Tijani AY, Ezugwu BO
The crave for evidence on the safety and efficacy of traditional medicines has led to this study on evaluation of the chemical composition, anti-proliferative and cytostatic effects of S. longepedunculata roots extract. The phytochemical investigation on the ethanol extract revealed the presence of tannins, saponins, saponin glycosides, alkaloids, flavonoids, cardiac glycosides, terpenes and steroids. The HPLC chromatogram showed seventeen peaks and the compounds with retention time of 4.891, 7.247, 8.745, 18.522 and 25.889 minutes corresponded to caffeic acid, rutin, ferulic acid, apigenin and quercetin respectively. Growth inhibition test of the roots extract on Sorghum bicolor seeds showed a significant (P<0.0001) result throughout the experiment against the control seeds (5% DMSO and Methotrexate). At 96h, percentage growth inhibitions for the seeds treated with 1mg/ml, 2mg/ml, 4mg/ml, 6mg/ml and 8mg/ml were 42.97%, 72.69%, 83.94%, 86.14%, 91.26% and 91.57% respectively. The results suggest the probable use of the plant in traditional medicine for the treatment of tumour related ailments and other diseases. However, further works using appropriate human cell lines are needed to find a novel drug.
Pages: 267-272 | 1417 Views 81 Downloads
Ogukwe CE, Okhale SE, Tijani AY, Ezugwu BO. Evaluation of chemical composition, cytostatic and anti-proliferative effects of ethanolic roots extract of Securidaca longepedunculata (Fresen). J Pharmacogn Phytochem 2015;4(4):267-272.