Journal of Pharmacognosy and Phytochemistry
Vol. 5, Issue 1 (2016)
Isolation and 1H-NMR identification of a tiliroside from Odontonema strictum (Acanthaceae)
Lokadi Pierre Luhata, Namboole Moses Munkombwe, Hanzooma Hatwiko
The dried leaves (310g) ofOdontonema strictum
(OSM) were poured and extracted in MeOH/DCM (1/1//v/v) for 48 hours to yield 25g of dry residue which were purified using chromatographic methods (vacuum liquid chromatography column (VLC), Sephadex LH-20 column and Thin Layer Chromatography (TLC)). Fractions containing flavonoids
were mixed for further purifications. Compound 1, yellow amorphous powder (8 mg) was re-dissolved in MeOH and developed in EtAOc/MeOH (2:1) to give an Rf value of 0.27 and mp
301-303Â°C. The compound reacted positively to HCl-Mg and Molish reactions. The 1 H-NMR (400 MHz, CDCl3) spectrum displayed the typical signals for tilirosides
. The presence of glycosidic flavonoids
may possibly contribute to the pharmacological properties of OSM.
Pages: 206-210 | 2312 Views 523 Downloads
Lokadi Pierre Luhata, Namboole Moses Munkombwe, Hanzooma Hatwiko. Isolation and 1H-NMR identification of a tiliroside from Odontonema strictum (Acanthaceae). J Pharmacogn Phytochem 2016;5(1):206-210.