Vincent Emile Rasamison, Harinantenaina Liva Rakotondraibe, Vahinalahaja Razafintsalama, Stephan Rakotonandrasana, Andriamalala Rakotondrafara, Michel Alain Ratsimbason, Elie Rafidinarivo
The ethanolic extract from stems and leaves of Diospyros gracilipes
Hiern showed moderate antimicrobial activity against Staphylococcus aureus
(9 mm), Klebsiella pneumonia
(7 mm) and Candida albicans
(11 mm) using the disc diffusion assay. Fractionation of the extract led to the isolation of lupenone, ursolic acid, corosolic acid, a mixture of 3Î±- and 3Î²- taraxerols, 3Î²-E
-coumaroyltaraxerol, pinocembrin, scopoletin, plumbagin and elliptinone as established by spectroscopic techniques. Plumbagin exhibited potent antimicrobial activity against the three test microorganisms with inhibitory zones ranging between 30 and 43 mm at 50 Âµg/disc, and 21 and 28 mm at 10 Âµg/disc. Plumbagin displayed significant cytotoxicity (IC50
=0.26 Âµg/mL) against HT-29 colon cancer cell line in the sulforhodamine B assay, whereas elliptinone showed weaker activity (IC50
=13.29 Âµg/mL) compared to plumbagin. The reported anti-progestational activity of plumbagin provides a rationale for the popular use of this plant as abortifacient.
Vincent Emile Rasamison, Harinantenaina Liva Rakotondraibe, Vahinalahaja Razafintsalama, Stephan Rakotonandrasana, Andriamalala Rakotondrafara, Michel Alain Ratsimbason, Elie Rafidinarivo. Chemical constituents from stems and leaves of Diospyros gracilipes Hiern and the antimicrobial and cytotoxic principles. J Pharmacogn Phytochem 2016;5(2):109-113.