P Vani, S Kistamma, K Srinivas Reddy, A Narshimha Reddy, Ch Mohan
The present paper reports the antibacterial, antioxidant activity and In vitro
propagation studies of a threatened plant, Gymnema sylvestre. G. sylvestre
which belongs to the family Asclepiadaceae is a slow growing perennial medicinal woody climber commonly called as â€œGudmarâ€. There is a growing demand for leaves of G. sylvestre
in the pharmaceutical trade due to its use as a remedy for diabetes and also as a tonic of the nerves and as a laxative. Micropropagation of this plant is often difficult and expensive. In the present study the antibacterial activity of extracts of different G. sylvestre
explants were performed against gram positive and gram negative bacteria by the disk diffusion method. The activities of the compounds were compared with standard strain for antibacterial properties of the imine base and its solvent extract evaluated and presenting in indicate that the compounds are active in exhibiting antibacterial role also carried out. G. Sylvestre
exhibited potent antioxidant activity by inhibiting DPPH free radicals which indicates the roots and leaves extract is very much source of natural antioxidant agent. In vitro
propagation is an alternative method of in vitro
propagation of the threatened and endangered plant which can aid its conservation. The nodal and intermodal explants were cultured on MS medium containing different concentration and combinations of growth regulators like BAP and IAA. Multiple shoots buds were regenerated successfully from the nodal explants which were efficiently rooted on half strength MS medium supplemented with IBA. The regenerated plantlets were successfully transferred to the glasshouse, acclimatized and transferred to the field.
P Vani, S Kistamma, K Srinivas Reddy, A Narshimha Reddy, Ch Mohan. Antibacterial activity, antioxidant activity and micropropagation of Gymnema sylvestre R.Br. a valuable medicinal plant. J Pharmacogn Phytochem 2016;5(2):207-210.