Journal of Pharmacognosy and Phytochemistry
Vol. 5, Issue 3 (2016)
Phytochemical and biological investigation of VLC fraction of Senna alata leaves
Repon Kumer Saha, Tirtha Nandi, Kamrun Nahar, Al Amin Talukdar and Pipon Chandra Nandi
The objective of this work was phytochemical and biological investigation of VLC fraction of Senna alata
leaves found in Bangladesh. The crude methanolic extract was fractionated by vacuum liquid chromatography by different five polar solvent. The resulted fractions were again fractionated by vacuum liquid chromatography by same five different polar solvent. We investigated the presence of polyphenols, flavonoids and other types of compounds by thin layer chromatography. In vitro
antioxidant effects were measured by DPPH scavenging assay. In vitro
anti- diabetic assay was carried out by glucose uptake in yeast cells. Disc diffusion assay was performed to show the antibacterial effect using gram positive, gram negative strains of bacteria and fungi. Receptor binding activities was performed by hemagglutination inhibition assay. VLC fraction of leaves contain flavonoids and other biologically active compounds. The extract showed antioxidant and anti-diabetic activity. The extract also showed antibacterial activities against several strains of bacteria. It also showed hemagglutination inhibition activities in human blood cells. Therefore, leaves of Senna alata
may be considered as a source of various health benefits.
Pages: 55-61 | 2392 Views 365 Downloads
Repon Kumer Saha, Tirtha Nandi, Kamrun Nahar, Al Amin Talukdar and Pipon Chandra Nandi. Phytochemical and biological investigation of VLC fraction of Senna alata leaves. J Pharmacogn Phytochem 2016;5(3):55-61.