Journal of Pharmacognosy and Phytochemistry
Vol. 5, Issue 4 (2016)
Phytochemicals and HPTLC studies of methanolic extract of different germplasms of Cordia dichotoma Frost f.
Pushpa H Nandedkar, Ramjan M Mulani
Objective: Most of the traditional medicinal plants in India are not scientifically validated. Scientific evaluation along with traditional knowledge is essential to obtain effective drugs for commercial purposes. To estimate the amount of important secondary metabolites and to establish the fingerprint profile of Cordia dichotoma
using high performance thin layer chromatography (HPTLC) technique. Methods: Phytochemical estimation were carried out with standard graph method and HPTLC studies were carried out in one solvent systems, which showed different Rf value. Results: Secondary metabolites profiling of the extract confirm about the presence of various phytochemicals. HPTLC finger printing of methanol extract of leaf bark and fruits revealed various peaks with Rf values in the range of -0.03 to 0.92. Conclusion: It can be concluded that different Rf value of various phytochemicals provide valuable clue regarding their polarity and selection of solvents for separation of phytochemicals. The study will help in future for identifying this plant for further research.
Pages: 06-12 | 1669 Views 122 Downloads
Pushpa H Nandedkar, Ramjan M Mulani. Phytochemicals and HPTLC studies of methanolic extract of different germplasms of Cordia dichotoma Frost f.. J Pharmacogn Phytochem 2016;5(4):06-12.