Author(s):

Dhriti Kapoor, Amandeep Rattan, Vandana Gautam and Renu Bhardwaj

Abstract: The present work was undertaken to analyze the effects of 24-epibrassinolide (10^-11M, 10^-9M and ₁₀^-7M) on growth, metal uptake, Na⁺ & K⁺ ions concentration, photosynthetic pigments, level of osmoprotectants, MDA content, level of antioxidants, protein content and activities of antioxidant enzymes in 7-days old seedlings of Raphanus sativus L. subjected to Hg metal (0.25 mM) stress. Seedling growth was improved by 24-epiBL treatments under heavy metal stress. Application of 24-EBL enhanced the level of osmolytes, antioxidants and also stimulated the activities of antioxidative enzymes. MDA content was found to enhance under stress conditions, which was reduced by treatment of EBL. Level of photosynthetic pigments and Na⁺ & K⁺ ions were also increased by the supplementation of EBL. 10^-9 and 10^-11 M concentrations of 24-EBL were proved to be the effective concentrations. In conclusion, treatment of 24-EBL helped in combating the metal stress by activating the various defence strategies of Raphanus sativus.

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