Nguyen Manh Cuong, Pham Ngoc Khanh, Vu Thi Ha, Tran Thu Huong, Ngo Thanh Tung, Nguyen Thi Cuc and Do Thi Thao
Indirubin-3â€²-oxime (IOX) is known to possess anticancer properties by inhibiting cell proliferation of various human cancer cell lines. In the present study, IOX was prepared directly from an indirubin-rich powder of Strobilanthes cusia
leaves through an eco-friendly procedure. IOX thus obtained was evaluated for the anticancer activity both in vitro
and in vivo
. IOX showed anti-cancer activity against various human cell lines (KB, HepG2, MCF-7, LU-1 and LLC); in particular IOX signiï¬cantly reduced the survival rate of human oral epidermoid (KB) and human lung cancer (LU-1) cells with IC50
values of 2.51 and 3.52 Âµg/ml, respectively. In the chronic in vivo
toxicity assay, IOX given per os
was essentially non-toxic to mice (LD50
> 12.0 g/kg). IOX antitumor activity in vivo
was assessed by use of the Lewis lung carcinoma (LLC) cells transplanted subcutaneously in the mice flank. Mice receiving for 40 days IOX at a daily oral dose of 200 mg/kg b.w. had their life span significantly prolonged by 50% while the tumor mass was significantly (P
<0.05) decreased by about 30% as compared to control animals. Since IOX was proved to suppress tumor growth in vivo by inhibiting tumor cell proliferation, it is a potential candidate for developing anticancer therapeutic agents.
Nguyen Manh Cuong, Pham Ngoc Khanh, Vu Thi Ha, Tran Thu Huong, Ngo Thanh Tung, Nguyen Thi Cuc and Do Thi Thao. Semi-synthesis of indirubin-3â€²-oxime from Strobilanthes cusia leaves, its acute and sub-chronic toxicity, in vitro and in vivo antitumor activity in Lewis lung carcinoma bearing mice. J Pharmacogn Phytochem 2016;5(4):292-301.