Author(s):

AP Attanayake, LDAM Arawwawala and KAPW Jayatilaka

Abstract: New drug leads from natural products either as pure compounds or as standardized herbal preparations provide a plethora of management opportunities in modern medicine. Coccinia grandis (Linn.) Voigt (Cucurbitaceae) has been widely used for the management of diabetes mellitus in Sri Lankan traditional medicine. The present investigation was to standardize the leaf extract of C. grandis through standard in vitro techniques. The determination of physicochemical parameters and antioxidant activity, analysis of selected heavy metals, phytochemicals and development of thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) fingerprints were carried out using standard protocols. The analysis of physicochemical parameters showed that the C. grandis leaves were consisted of 14.2% moisture, 19.5% total ash, 13.3 % water soluble ash, 1% acid insoluble ash. In contrast, selected heavy metals were absent in the plant material. TLC and HPLC fingerprints clearly demonstrated the proportional differences of these chemical constituents. In conclusion, the quality control parameters obtained from this study can be used as reference standards for the leaf of C. grandis of Sri Lankan origin.

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