Author(s):

Ruwani Punyakanthi Hewawasam, Kamani Ayoma Perera Wijewardene Jayatilaka and Chitra Pathirana

Abstract: The main objective of this study was to investigate the antioxidative effect of Asteracantha longifolia extract against carbon tetrachloride (CCl₄) and paracetamol induced oxidative damage in ICR mice. Hepatotoxicity was induced by a single intraperitoneal dose of CCl₄ (0.5 ml/kg) and paracetamol (300 mg/kg, orally) after a 16 h fast. An aqueous extract of Asteracantha was administered to mice on a pre and post-treatment basis. A significant increase in glutathione peroxidase and a significant reduction in glutathione reductase and glutathione-S-transferase concentrations were observed in CCl₄ and paracetamol control groups compared to normal control. A significant increase in malondialdehyde formation was observed in the liver cytosol and serum in both toxin control groups. Pre- and post-treatment with Asteracantha significantly improved the antioxidant enzyme profiles against CCl₄ and paracetamol control groups. Overall results indicate that aqueous extract of Asteracantha longifolia could ameliorate oxidative stress induced by CCl4 and paracetamol in ICR mice.

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