Frantz FranÃ§ois-Haugrin, Max Monan, Emmanuel Nossin, Juliette Smith-Ravin and Odile Marcelin
Isolation and identification of the main flavonoid from petals of Talipariti elatum Sw. (Hibiscus elatus Sw), comparison of antioxidant properties, determination of total phenolic content of the flower.
Method: The main flavonoid was precipitated using 1, 2 - dimethoxyethane, followed by two serial recrystallizations. Analysis of the precipitate was done with UV spectrum, MS-NMR 1H and MS-NMR 13C spectroscopy. Antioxidant activity was carried out using 1,1- diphenyl -2-picrylhydrazyl (DPPH) and Î²-carotene.
Results: The main flavonoid of the petals is gossypetin-3â€™-O-glucoside. Until now Cuban research workers argued that major flavonoid was gossypitrin (gossypetin-7-O-glucoside). DPPH test leads to an EC50 value of 0,11 mg/L, however Î²-carotene bleaching test (BCB test) didnâ€™t give positive results. Total phenolic content of ethanolic extract from dried flower is 163 Â± 5 mg GAE/g.
Conclusion: Talipariti elatum Sw. is a high source of natural antioxidants. Precedent publications revealed also the presence of various flavonols or flavones (quercetin, rutin). Antimicrobial, anti-hepatotoxic, anti-catarrh, anti-cough, anti-asthma properties of this specie show it great interest in healthâ€™s range.
Frantz FranÃ§ois-Haugrin, Max Monan, Emmanuel Nossin, Juliette Smith-Ravin and Odile Marcelin. Antioxidant activity of an isomer of gossypitrin (gossypetin-3â€™-O-glucoside) isolated in the petals of Talipariti elatum Sw., and determination of total phenolic content of the total flower. J Pharmacogn Phytochem 2016;5(5):200-208.