Journal of Pharmacognosy and Phytochemistry
Vol. 5, Issue 6 (2016)
Isolation and Purification of Lectin from Soybean (Glycine Max) Mahabeej Js-335
Amit T Bondar, Shobha B Tole and Dr. Shivanand M Patil
Plant lectins have a wide spectrum of biological significance. In this project work the isolation and purification of the lectins (SBL) from the Soybean (Glycine max) Mahabeej JS-335 have been reported. The lactose binding lectins were purified from the Soybean seeds by performing affinity chromatography using a lactamil sephadex 100G. Affinity products were dialysed against PBS and SDS-PAGE was done to identify the fractions â€˜molecular weight. This work explored different techniques of protein (lectin) purification and describing their characteristics.
Pages: 89-92 | 2692 Views 576 Downloads
Amit T Bondar, Shobha B Tole and Dr. Shivanand M Patil. Isolation and Purification of Lectin from Soybean (Glycine Max) Mahabeej Js-335. J Pharmacogn Phytochem 2016;5(6):89-92.