Journal of Pharmacognosy and Phytochemistry
Vol. 5, Issue 6 (2016)
Determination of hypericin in St. Johnâ€™s wort by derivative spectrophotometry
Sum of hypericine content in St. Johnâ€™s Wort was determined by second order derivative spectrophotometry without using any separation or background correction technique and reagent. Calibration curves were constructed for the 5â€“25 Î¼g mlâˆ’1 concentration range. As a reference method, reversed phase high performance liquid chromatographic procedure was developed. Commercially available samples of St. Johnâ€™s Wort were analyzed by the two methods and the results were statistically compared.
Pages: 249-253 | 1580 Views 182 Downloads
Dmitriy Moiseev. Determination of hypericin in St. Johnâ€™s wort by derivative spectrophotometry. J Pharmacogn Phytochem 2016;5(6):249-253.