Journal of Pharmacognosy and Phytochemistry
Vol. 5, Issue 6 (2016)
Molecular characterization, lipid analysis and GC-MS determination of bioactive compounds identified in a West African strain of the green alga Oedogonium (Chlorophyta)
Adesalu TA, Temenu TO and Julius ML
In this study, the species of Oedogonium
was subjected to two weeks batch culturing using Boldâ€™s Basal Medium (BBM).The DNA of the pure culture obtained was extracted using DNeasyÂ® Plant Mini Kit following the protocols, conserved DNA regions of chloroplast genome corresponding to SSU/18S rDNA and Rbcl were amplified and Polymerase Chain Reaction (PCR) product was sequenced. Based on morphology and DNA barcoding, isolates were identified as Oedogonium tenerum
for SSU/18S rDNA and Oedogonium cardiacum
Wittrock ex Hirn (Chlorophyta) for Rbcl. Lipids analysis and the active compounds of Oedogonium
strain were evaluated using Agilent 7890A Gas Chromatography (GC) system and Agilent 5975C inert Mass Spectrometer Detector. Analysis of the sample lipids showed 12.98% lipid content while GC-MS analysis of hexane extract of Oedogonium
strain (KU680468 (SSU/18S rDNA); KU680573 Rbcl) revealed the existence of Eicosane, 1-Nonadecene, Tricosane, Heneicosane and Nona hexacontanoic acid adding up to 70.27%. The minor constituents include Butylated hydroxytoluene, Nonadecane, 1-Docosene adding up to 29.3%. The importance of some of these components in medicine includes anti-viral and anti-oxidant properties. Other importance of these hydrocarbons are found in food preservatives and majorly in the petrochemical industry for the production of candles, biopesticides (insect pheromones), paraffin wax and other by-products of petroleum. The result implied a closely related but unique strain which is probably endemic to West Africa and Nigeria in particular as other strains are from North America.
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Adesalu TA, Temenu TO and Julius ML. Molecular characterization, lipid analysis and GC-MS determination of bioactive compounds identified in a West African strain of the green alga Oedogonium (Chlorophyta). J Pharmacogn Phytochem 2016;5(6):01-06.