Journal of Pharmacognosy and Phytochemistry
Vol. 6, Issue 1 (2017)
HPLC analysis of saponins in Achyranthes aspera and Cissus quadrangularis
Tamanna Talreja, Mangesh Kumar, Asha Goswami, Ghanshyam Gahlot, Surendra Kumar Jinger and Tribhuwan Sharma
Reversed phase high performance liquid chromatography (RP-HPLC) with UV detector was done for the analysis of four saponins of Achyranthes aspera
and Cissus quadrangularis
. The separation of filtered plant extracts as well as a mixture of authentic standard saponin samples of Gitogenin, Diosgenin, Hecogenin, Kryptogenin was done within 25 min. Running conditions included: injection volume 15Âµl; mobile phase: acetonitrile: water (40:60) running time 25 min., flow rate 1 ml/min; and detection at 203nm. Identifications of specific saponins are made by comparing their retention times with those of the standards. Three saponins in Achyranthes aspera
namely Gitogenin, Diosgenin, Kryptogenin and two saponins namely Diosgenin, Kryptogenin in Cissus quadrangularis
were detected by HPLC.
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Tamanna Talreja, Mangesh Kumar, Asha Goswami, Ghanshyam Gahlot, Surendra Kumar Jinger and Tribhuwan Sharma. HPLC analysis of saponins in Achyranthes aspera and Cissus quadrangularis. J Pharmacogn Phytochem 2017;6(1):89-92.