Journal of Pharmacognosy and Phytochemistry
Vol. 6, Issue 1 (2017)
Phytochemical screening, RP-HPLC and FTIR Analysis of Kappaphycus alvarezii (Doty) Doty EX P.C Silva: Macro red algae
Sumayya SS and K Murugan
Sea weeds are immense source of bioactive molecules for the exploration of novel drugs. The study was aimed to identify the phytochemicals present in the red algae Kappaphycus alvarezii. The sample was collected from the sea coast of Thoothukudi, Tamil Nadu. The different solvent extracts from K. alvarezii was found to be rich in phenols and carbohydrate. The carbohydrate content of the methanolic extracts of algal extract was 18.26 mg/g.f.wt, phenol 23.01mg GAE standard, gallic acid equivalent/100 g dry wt. The phytochemical analysis revealed the presence of carbohydrate, protein, alkaloids, glycosides, flavonoids, steroid, phenolic compounds and absence of tannin in the extracts. Also K. alvarezii extracts revealed macromolecules contents such as carbohydrate contents of 18.26 g, protein was 11.49 g and fat content of 1.01 g /100 g of dry wt. RP-HPLC analysis showed the presence of a pool of phenolic acids with higher level of sinapic acid followed by phloroglucinol. Subsequently, the different solvent extracts of K. alvarezii were subjected to fourier transform infrared spectroscopy for the analysis of functional groups. The results based on spectral data of FTIR revealed the presence of aliphatic constituents containing carbon, ketones, alkyl halides and hydroxyl groups. Thus, the phytochemical analysis of the algae showed immense potential values in terms of primary and secondary metabolites and therefore it can be used as functional and pharmaceutical food.
Pages: 325-330 | 1387 Views 135 Downloads
Sumayya SS and K Murugan. Phytochemical screening, RP-HPLC and FTIR Analysis of Kappaphycus alvarezii (Doty) Doty EX P.C Silva: Macro red algae. J Pharmacogn Phytochem 2017;6(1):325-330.