Journal of Pharmacognosy and Phytochemistry
Vol. 6, Issue 1 (2017)
UV-Vis scanning coupled with PCA as an alternative method for phytochemical screening of natural products â€“ Costus Igneus leaf metabolites
S Thiruchenduran, K Uma Maheswari, TNVKV Prasad, B Rajeswari and WJ Suneetha
Utilization of medicinal plants to discover new drugs and to invent nutraceuticals and functional foods is the current trend for natural product researchers and food technologists. Availability of cost effective and reliable methods to identify the functional groups and bioactive molecules from natural products are scarce. In this study the patterns of absorbance at different wavelengths by the bioactive compounds were detected using principal component analysis of the absorbance data of extracts of Costus igneus plant leaf. Thin layer chromatography method and qualitative phytochemical screening chemicals methods were done simultaneously. Principal component analysis depicted significant correlation (p<0.5) between wavelengths 410nm-670nm, 330nm-350nm-370nm-410nm and 210nm-290nm. Principal component analysis and consequent literature search indicated the presence of flavonoids, catechins, chlorophyll a and b and antioxidant polyphenolic compounds. These results correlates with qualitative phytochemical screening and thin layer chromatography. UV-Vis scanning coupled with principal component analysis is a cheap and reliable method for preliminary screening of natural products for bioactive flavonoids and chlorophylls.
Pages: 411-416 | 1226 Views 69 Downloads
S Thiruchenduran, K Uma Maheswari, TNVKV Prasad, B Rajeswari and WJ Suneetha. UV-Vis scanning coupled with PCA as an alternative method for phytochemical screening of natural products â€“ Costus Igneus leaf metabolites. J Pharmacogn Phytochem 2017;6(1):411-416.