Abstract:
The
present research study focused on identification and quantification of γ-tocopherol in
n-hexane and petroleum ether seed extracts of
Cucurbita pepo (CP),
Cucumis melo (CM)
, Cucumis sativus (CS) belonging to family Cucurbitaceae by High Performance Liquid Chromatography (HPLC).
n-Hexane and petroleum ether extracts were prepared using soxhlet extraction and γ-tocopherol was identified and quantified using HPLC analysis with ultraviolet/visible (UV–vis) detection. The chromatographic conditions for HPLC analysis followed were: column, reverse-phase C-18, 5µ, 250x4.6mm; column temperature, 25°C; mobile phase, methanol: acetonitrile 60:40(v/v); flow rate, 1.0ml/min; detection wavelength, 292nm; run time, 15minutes.
n-Hexane andpetroleum ether seed extracts of
Cucurbita pepo,
Cucumis melo and
Cucumis sativus were determined to contain 0.2900%w/v, 0.6666%w/v, 0.9162%w/v and 0.5093%w/v, 0.7781%w/v, 0.5564%w/v γ-tocopherol respectively. From the results obtained it can be concluded that seeds of
Cucurbita pepo,
Cucumis melo and
Cucumis sativus are good sources of γ-tocopherol which justifies their medicinal and commercial uses.
Gaurav M Doshi and Preeti P Kanade. Identification and quantification of γ-tocopherol in Cucurbita pepo, Cucumis melo and Cucumis sativus seeds extracts by high performance liquid chromatography. J Pharmacogn Phytochem 2017;6(2):192-196.