Journal of Pharmacognosy and Phytochemistry
Vol. 6, Issue 4 (2017)
Effect of naringinase enzymatic treatment on the bitter compound naringin in fresh juice of â€œBibila sweetâ€ oranges
G Oshadie De Silva, RAUJ Marapana and Ranmalie Manawaduge
Enhanced bitterness in citrus juices is one of the common quality problem in beverage industry. Limonin and naringin are the two main responsible compounds for the bitterness. The aim of this study is to determine the effectiveness of adding naringinase enzyme on reducing naringin in â€œBibila sweetâ€ oranges to enhance its quality. Naringinase can decompose naringin in to less bitter precursors and it can be inactivated by heating up to 90 0
C. Three enzyme concentrations as 1.0 g/L, 0.5 g/L and 0.25 g/L with two incubation time periods of 2 and 4 hours used at constant incubation temperature of 500
C were used as the variables. The concentration of Naringin was analyzed using Davis semi quantitative method and Ultra High Performance Liquid Chromatography. The optimum reduction can be obtained by adding 1.0g/L naringinase enzyme at 50 0
C with 4 hours of incubation period with 86% reduction percentage.
Pages: 174-178 | 2033 Views 579 Downloads
G Oshadie De Silva, RAUJ Marapana and Ranmalie Manawaduge. Effect of naringinase enzymatic treatment on the bitter compound naringin in fresh juice of â€œBibila sweetâ€ oranges. J Pharmacogn Phytochem 2017;6(4):174-178.