Author(s):

K Kannabiran, K Rajamani, J Suresh, R John Joel and D Uma

Abstract:
The study was conducted to induce variability through mutation breeding using physical and chemical mutagens in periwinklecv. Local. The physical mutagen i.e., gamma radiation dose ranging from 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 and 60 kR and chemical mutagen i.e., EMS ranging from 10, 20, 30,40, 50 and 60 mM. The leaf and root samples are collected from the mutated plants (M₂) having morphological variation plant height, flower color, leaf color and yield characters. The selected mutants of 40kR, 30mM were exposed for alkaloid estimation through High Performance Liquid Chromatography. The putative mutants were selected based on chromatographic pattern with having highest alkaloids are present compared to control. The higher vincristine content was observed in 40 kR (0.37 %), followed by 30mM (0.35%). The physical and chemical mutagens contributed to variabilities in alkaloid contents in periwinkle.The effect of physical mutagen i.e., gamma radiation and on EMS on phytochemical analysis from the mutated periwinkle leaves. The results showed that the different concentrations used for induced mutagenesis in periwinkle 40 kR and 30mM can be highly beneficial to create variability.

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