Journal of Pharmacognosy and Phytochemistry
Vol. 6, Issue 6 (2017)
Biochemical characterization of proteases isolated from the latex of Tabernaemontana divaricata L. and Carissa carandas L.: Their role in hemostasis
Author(s):
Syeda Hajira Banu, Nagashree S, Latha BV and Mukunda Chethankumar
Abstract:
In the present investigation, comparative profile of proteases isolated from the latex of
Tabernaemontana divaricata L. and
Carissa carandas L. of Apocynaceae and their role in hemostasis was studied. The crude enzyme was prepared from the latex of
Tabernaemontana divaricata L. and
Carissa carandas L. by repeated freeze-thaw followed by centrifugation. The protein yield was 30mg/ml for
Tabernaemontana divaricata L. and 66mg/ml for
Carissa carandas L. Sephadex G-75 fractionation yielded major peak TDP4 (19kDa) from
Tabernaemontana divaricata L. and CCP1 (22kDa) from
Carissa carandas L. with significant proteolytic activity. The enzymes showed dose-dependent caseinolytic, fibrinogenolytic, blood and plasma clot activities. TDP4 and CCP1 exhibited optimum pH at 6.8 and 7.8 & temperature at 40
0C ±2
0C and 25
0C ±2
0C respectively. TDP4 hydrolyzed blood clot, while CCP1 hydrolyzed plasma clot. Caseinolytic activity of TDP4 and CCP1 increased by 4-folds upon β-mercaptoethanol treatment and inhibited by iodine suggesting that the enzymes are serine proteases.
Pages: 06-09 | 3990 Views 652 Downloads
Syeda Hajira Banu, Nagashree S, Latha BV and Mukunda Chethankumar. Biochemical characterization of proteases isolated from the latex of Tabernaemontana divaricata L. and Carissa carandas L.: Their role in hemostasis. J Pharmacogn Phytochem 2017;6(6):06-09.