Author(s):

S Anbu, J Padma, K Punithavalli and P Saranraj

Abstract: The present study was aimed to formulate growth media using fruits peel waste materials such as Pine apple, Mango, Jack fruit, Green Banana, Yellow Banana, Sweet Lime and Pomegranate. The fresh fruits were collected from local market of Tirupattur, Tamil Nadu and the peels were used for the study. The peels were air dried, grinded into fine particles using mortar and pestle and then sieved with 1mm sieve size. Fungi isolates (Aspergillus niger, Rhizopus stolonifer and Penicillium chrysogenum) were isolated from spoilt bread and orange using potato dextrose agar (PDA) and identified. About 4.0 grams of fruit peel wastes were added into the 100 ml of distilled water and sterilized by autoclaving at 121°C for 15 minutes. After Serilization, the fruit peel waste broths were cooled and then one ml of industrially important fungal inoculums was added. The inoculums added broths were incubated at room temperature for 3 days. The presence/absence of the fungi growth was visually observed.
A. niger growth was recorded in the medium containing Pine apple, Mango, Jack fruit and Green banana. The A. niger growth was not recorded in the medium containing Yellow banana, Sweet lime and Pomegranate. R. stolonifer growth was observed in the medium which contains Pine apple, Mango, Sweet lime and Pomegranate. The R. stolonifer growth was not recorded in the medium containing Yellow banana, Jack fruit and Mango. However, the P. chrysogenum growth was recorded in the medium which contains Pine apple, Mango, Sweet lime and Pomegranate. The P. chrysogenum growth was not recorded in the medium containing Yellow banana, Jack fruit and Mango.

Pages: 426-428  |  3698 Views  1599 Downloads

Download (525KB)