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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 6, Issue 6 (2017)

Isolation, partial purification, product formation and characterization of β- glucosidase from spikes of Hordeum vulgare

Author(s):

Suman Tiwari and OP Verma

Abstract:
β-glucosidase (EC 3.2.1.21) was extracted from spikes of Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and Sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 13-85KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC. Bioethanol was produced from spikes of Hordeum vulgare.

Pages: 1657-1659  |  1092 Views  277 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Suman Tiwari and OP Verma. Isolation, partial purification, product formation and characterization of β- glucosidase from spikes of Hordeum vulgare. J Pharmacogn Phytochem 2017;6(6):1657-1659.

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