Journal of Pharmacognosy and Phytochemistry
Vol. 6, Issue 6 (2017)
Isolation, partial purification, product formation and characterization of β- glucosidase from spikes of Hordeum vulgare
Author(s):
Suman Tiwari and OP Verma
Abstract:
β-glucosidase (EC 3.2.1.21) was extracted from spikes of
Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and Sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 13-85KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC. Bioethanol was produced from spikes of
Hordeum vulgare.
Pages: 1657-1659 | 1196 Views 304 Downloads
Suman Tiwari and OP Verma. Isolation, partial purification, product formation and characterization of β- glucosidase from spikes of Hordeum vulgare. J Pharmacogn Phytochem 2017;6(6):1657-1659.