Brinjal is popular vegetable crop grown in the tropics and subtropics. Fruit and shoot borer (FSB) Leucinode orbonalis
is most serious and destructive pest causing heavy yield losses. Agrobacterium
mediated genetic transformation was performed in two brinjal cultivars viz
RHRB-35 and Manjarigota with Cry1Aabc
gene. Several factors influencing transformation efficiency such as type of explants, Agrobacterium
cell density, infection time, pre-culture duration, co-cultivation duration, overgrowth control antibiotics have been evaluated. Combination of cefotaxime and carbenicillin at 250 mg/l concentration effectively control Agrobacterium
overgrowth. Two week old Cotyledon and three week old hypocotyls explants precultured for 2 days and then infected with Agrobacterium
= 0.2 to 0.4) for 1 min showed maximum transformation efficiency. Among the two brinjal cultivars, RHRB-35 exhibited maximum transformation efficiency (8.44 %). The transgene integration into putatively transformed brinjal eggplant genome was confirmed by PCR analysis using gene specific primers.
Sonali Habde, Ashok Jadhav, Pawan Kulwal and Bhausaheb Pawar. Development of fruit and shoot borer resistant transgenic brinjal with Cry1Aabc gene: An assessment of factors influencing transformation efficiency. J Pharmacogn Phytochem 2017;6(6):2009-2013.