Vinay Kumar NM, Riaz Mahmood, V Krishna, Ravishankar B and Sudhesh L Shastri
Currently, plant based drug production demand has increased for the curing of various diseases. This study investigates the phytochemical constituents screening and antibacterial property of Gardenia gummifera
stem bark and leaf extracts. Preliminary phytochemical screening of various extracts using standard methods revealed the presence of alkaloids, phenolic compounds, terpenoids, tannins, glycosides and saponins. The antibacterial efficiency of the extracts were screened by agar well diffusion method against human pathogens viz, Staphylococcus aureus, Streptococcus pneumoniae
, Vibrio cholera, Pseudomonas aeruginosa
, Salmonella typhi
and Klebsiella pneumoniae
. Ethanol stem bark extract showed the pronounced antibacterial effect with 12.67Â±0.33mm and 12.33Â±0.33mm
inhibition zone against S. typhi
respectively and leaf extract showed 12.33Â±0.33mm and 12.83Â±0.60mm
inhibition zone against S. typhi and S. aureus
respectively. Leaves ethanol extract exhibited highest inhibitory activity against S, aureus
with MIC value of
8.33 Î¼g. The obtained results have supported the traditional claim of Gardenia gummifera
stem bark and leaves to control microbial infection.
Vinay Kumar NM, Riaz Mahmood, V Krishna, Ravishankar B and Sudhesh L Shastri. Evaluation of antibacterial activity from stem bark and leaf extracts of Gardenia gummifera Linn. J Pharmacogn Phytochem 2017;6(6):2026-2030.