Author(s):

Rahul V Kadam, Dr. SW Jadhav and PB Abhang

Abstract: The present investigation entitled, “Molecular characterization of MPKV biofertilzer” was focused on molecular diversity among the bacterial spp. by employing DNA fingerprinting technique, Random Amplified Polymorphic DNA. DNA fingerprinting at different bacterial spp. were carried out using RAPD analysis. To find out the relatedness and diversity based on polymorphism in RAPD profile. The total genomic DNA of bacterial strains was isolated by following the method given by Ivanova et al.; Ten RAPD primers of OPB series used for RAPD analysis, ten primers viz., RBa-01, RBa-02, RBa-03, RBa-04, RBa-05, RBa-06, RBa-07, RBa-08, RBa-09and RBa-10. RBa-01, RBa-04 and RBa-06 yielded good and different banding pattern. In the present study the size range of the bands amplified with selected 10 random primers was found in between 5500bp to 250bp when compared with DNA Molecular weight marker of 1kb. Thus, on the basis of variation of size number and intensity of bands, Rhizobium species were grouped into two groups: group I contains Bradyrhizobium japonicum, Rhizobium loti and Rhizobium spp. and group II contains Rhizobium meliloti, Rhizobium leguminasorum which depicts the diversity between them. This study demonstrates that the RAPD markers are useful in assessing diversity at genetic level rather than morphological markers which are subjected to environmental fluctuations.

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