Journal of Pharmacognosy and Phytochemistry
Vol. 7, Issue 1 (2018)
Phyto-chemical Characterization of the Leaf extracts of Terminalia catappa L. (Combretaceae) Using Ultra violet-Visible, Fourier transform infrared and Gas chromatography-Mass Spectroscopic techniques
Chioma Nnennaya Chinaka, Kenneth Maduabuchi Ezealisiji and Rukevwe Elohor Akpofure
Although the African herbal medicine have achieved unequivocal success as an ethno medicine yet they suffer many setbacks in their commercial value and global acceptance as a result of poor regulation and standardization. This study aims to characterize the phyto- chemical constituents present in the n-hexane and dichloromethane leaf extracts of Terminalia catappa
using chromatographic and spectroscopic methods. The crude n-hexane and dichloromethane extracts were analyzed using ultra-violet spectroscopy (UV-2500PC series), Fourier Transform Infrared spectroscopy (FTIR) model 8400S and GC-MS (model â€“QP 2010 plus Spec). The identification of compounds was done using NIST Ver. 2.0 Year 2005 library. The biological activity are based on Dr Dukeâ€™s phytochemical and ethno-botanical databases. Peaks corresponding to chalcones, simple phenols, carotenoids and chlorophyll were observed in the UV-Visible spectroscopy. The FTIR revealed functional groups such as alkanes, alkenes, alcohols and ethers. The GC-MS revealed phyto-compounds such as n-Hexadecanoic acid, octadecanoic acid, and chloroacetic acid present in the dichloromethane extract and stearic acid, oleic acid, n-Heptadenoic acid, in the n-hexane extract. T
he presence of these phytochemicals especially long chain fatty acids and their percentage occurrence may provide proper identification and explain their usage in ethno-medicine.
Pages: 2017-2023 | 1869 Views 773 Downloads
Chioma Nnennaya Chinaka, Kenneth Maduabuchi Ezealisiji and Rukevwe Elohor Akpofure. Phyto-chemical Characterization of the Leaf extracts of Terminalia catappa L. (Combretaceae) Using Ultra violet-Visible, Fourier transform infrared and Gas chromatography-Mass Spectroscopic techniques. J Pharmacogn Phytochem 2018;7(1):2017-2023.