Author(s):

SK Khatik, K Mathur, GK Bagri, R Kumari, DK Bagri and DL Bagdi

Abstract: Thirteen different natural, semi-synthetic and synthetic media were evaluated for its growth and sporulation. Richard's Agar, Asthana and Hawker's Agar and Sabouraud’s agar were supported good growth and sporulation. Of the eight different natural products evaluated for growth and sporulation of G. trianthemae, wheat bran Agar supported the maximum mycelial while chickpea flour agar supported maximum sporulation. Of the seven different micronutrient evaluated at lower and higher doses, the maximum mycelial growth (76.20 mm) occurred with Ferric Nitrate at 500 mg/lit, while the maximum sporulation was found with 25 mg/lit of ammonium molybdate. The maximum mycelial growth and sporulation occurred at 35°C and 30°C respectively. The maximum dry mycelial weight of G. trianthemae were observed at the pH 5.5, while in liquid medium sporulation was low.

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