Author(s):

Jan Sher, Gul Jan, Cai zhiquan, Muhammad Israr, Farzana Gul, Siraj Khan, Muddaser Shah and Shakir Ullah

Abstract: We investigated the scientific base for its traditional use in pain, inflammation, antioxidant and phytochemicals potential of Dryopteris blanfordii. The powdered plant was extracted by the method of cold maceration using (methanol, ethanol and aqueous) as solvents. The acetic acid induced writhing test model was used for analgesic activity. Anti-inflammatory activity was evaluated by carrageenan-induced mice paw edema. For antioxidant activity we used 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay method respectively. In qualitative analysis, the phytochemical compounds such as carbohydrates, alkaloids, phenols, flavonoids, saponins, tannins and quinine were screened by using standard methods. In analgesic activity after the injection of ethanolic extract at a dose of 300mg/kg the writhing was reduced to (21±0.13) /5 minutes and showed 45% inhibition, compared with standard drug aspirin which reduced to (14±0.21) /5 minutes and having 40.43% inhibition. The anti-inflammatory activity at a dose of 300 mg/kg during drug administration after four hours showed significant different which compared with standard drug diclofenac sodium (10mg/kg) and 100, 200mg/kg. High dose at 300mg/kg reduced (1.04±0.03) after the 4th hours and standard drug diclofenac sodium reduced (0.08±0.04) after 4th hours. The maximum rate of inhibition of antioxidant was observed in the methanolic extract. In the qualitative analysis of the ethanol extract showed highest amount of phenols. We concluded that the D.blanfordii plant showed high potential of inflammation, antioxidant and biochemical compounds.

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