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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 7, Issue 3 (2018)

Assessment of bioactive compounds in Brassica juncea using chromatographic techniques

Author(s):

Anubhuti Sharma and PK Rai

Abstract:
The investigation was carried out to determine the phytochemical components from methanolic extract of Indian mustard using High performance liquid chromatography (HPLC) and gas-chromatography mass spectrometry (GC-MS). GC-MS analysis of the extract led to the identification of more than 5 compounds in all the genotypes together. The major compounds in these mustard genotypes were found to be methallyl cyanide; allyl isothiocyanate; Oxime-methoxy-phenyl; 1-butene-4-isothiocyanato; 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl; 2-Furancarboxaldehyde; 5-(hydroxym-ethyl)-methyl-beta-d-galactopyranoside. Moreover, the genotype also showed allyl isothiocyantes in small percentage. Fatty acid analysis of Indian mustard also showed the presence of good amount of oleic acid, linolic acid and palmitic acid. The detection of these compounds in seeds of mustard genotypes highlights the pharmaceutical properties of this crop. The results inferred that these genotypes contain variable patterns of bioactive compounds and could be used as natural antioxidant source for various herbal formulation as anti-inflammatory, analgesic, antipyretic, cardiac tonic and antiasthamatic.

Pages: 1274-1277  |  1634 Views  648 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Anubhuti Sharma and PK Rai. Assessment of bioactive compounds in Brassica juncea using chromatographic techniques. J Pharmacogn Phytochem 2018;7(3):1274-1277.

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