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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 7, Issue 3 (2018)

Development and molecular characterization of transgenic Pigeon pea carrying cry2Aa for pod borer resistance

Author(s):

Tirthkar Meera Baburao and Bhat Sumangala

Abstract:
Pigeonpea is an important legume which belongs to the family Fabaceae. It is an excellent source of seed protein content of about 21 per cent. Pod borer or Helicoverpa armigera a lepidopteron insect is the most serious and widespread pest of pigeonpea. Two popular varieties Asha and BSMR736 were used for tissue culture independent Agrobacterium mediated transformation. The plant to row progeny screening of 216 primary transformants in T1 generation using cry2Aa specific PCR assay, identified seventeen transformants. Insect bioassay and Cry protein estimation using ELISA conducted simultaneously showed larval mortality ranging from 20% to 70% and the Cry2Aa content from 0.04 to 1.45 μg/g of fresh leaf tissue. Reduction in larval weight and length ranged from 13.32% to 47.13% and 42.33% to 64.95% respectively. Southern blot analysis indicated the single copy integration in selected events. The selected transgenic events can serve as resource for the development of pyramided transgenic pigeonpea in future.

Pages: 1581-1585  |  1032 Views  325 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Tirthkar Meera Baburao and Bhat Sumangala. Development and molecular characterization of transgenic Pigeon pea carrying cry2Aa for pod borer resistance. J Pharmacogn Phytochem 2018;7(3):1581-1585.

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