Author(s):

Shashi Kant, Jagdeep Singh Dua and Viney Lather

Abstract: In the present work deals with the extraction of methanolic extract of Blepharis edulis and investigation of antidiabetic effects in male Wistar albino rats. Intraperitoneal injection of streptozotocin solution (60 mg/kg body weight) was given to the fasted animals to induce diabetes. The samples were collected to analyze blood glucose level and plasma insulin levels at the end of the study. Blood glucose levels were measured using glucometer and enzyme-linked immunosorbent assay (ELISA) method was used for the determination of insulin levels. Insulin level, triglyceride level, cholesterol level, low-density lipoproteins (LDL) level, serum glutamic oxaloacetic transaminase (SGOT) level and serum glutamic pyruvic transaminase (SGPT) level were also examined at the end of the study. The liver and pancreas of the treated animals were isolated for histopathology examinations. The phytochemical screening of Blepharis edulis methanolic extract indicated the presence of alkaloid, flavonoids and phenolic groups. The results of in vivo study showed a significant decline in fasting blood glucose levels. The extract at high dose significantly (p< 0.05) normalized plasma insulin level. The results revealed that the methanolic extract significantly (p< 0.05) normalized plasma lipid status and decreased cholesterol, triglyceride and LDL levels. A significant (p< 0.05) increase in SGPT level was observed. However the effect on SGOT level was non-significant. The histopathologic study results revealed that the treatment of animals with pure glibenclamide (10 mg/kg) and Blepharis edulis plant extract showed a decreased pattern of liver enzymatic activities.

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