Author(s):

Krishan Kumar Singh and Shiv Pratap Singh

Abstract: The rapid process for development of identical offspring is micropropagation. In this technique the plants grow from meristematic tissue or somatic cells of superior plants using suitable nutrient media under controlled environmental conditions. Micropropagation in Guava, was successfully done on the variety Allahabad safeda. After that micropropagation progress has been made in the different areas via in vitro culture of guava. MSO or MS0 (MS-zero) was mostly used as mineral medium for culturing Psidium species. The Woody Plant Medium (WPM) was more suitable for micro propagation of some guava genotypes. Starting material for guava culture (in vitro) is growing shoot tips or nodal segments as explants (1.0-3.0 cm size) collected during in spring. Browning of the explant and culture medium is mostly obstruct in culturing of woody plants, which is remove by avoidance of toxic substances in the medium in guava. Cytokinin (6-Benzylaminopurine) is the most frequently used for guava micropropagation. Use of dual auxins (IBA & NAA) in compare to IBA followed by NAA, resulted higher frequency of rooting, which is important for successful survival of plant. In guava, acclimatization has been experienced by gradually decreasing the relative humidity.

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