Author(s):

Raghavendra S, Satish D, Dileep Kumar Masuthi, Padmanabha K, Ramesh CK and Vadlapudi Kumar

Abstract: L-dopa (3, 4-dihydroxy L-phenylalanine) is a neurotransmitter precursor being used for symptomatic relief of Parkinson’s disease. It is one of the most highly active allele chemicals present in Mucuna species. L-Dopa is produced via the oxidation of tyrosine by the copper containing enzyme tyrosine hydroxylase. The effect of elicitors and precursor molecules on the activity of tyrosine hydroxylase and their effect on the production of L-Dopa in cell suspension cultures of Mucuna prurita Hook were studied. Callus cultures were initiated on MS medium supplemented with BAP and IAA. The callus was transferred to MS liquid medium supplemented with BAP, IAA and elicitors methyl jasmonate, chitin, pectin, yeast extract and also a precursor L-tyrosine at different concentrations. Compared to the control, several fold increase in the activity of tyrosine hydroxylase was observed. With the progressive increase in the activity of tyrosine hydroxylase also, several fold increase in L-Dopa production was observed in the elicited and precursor fed cell suspension cultures. Compared to elicitor treatments, precursor feeding had more impact on L-Dopa production and on the activity of tyrosine hydroxylase.

Pages: 179-185  |  1600 Views  363 Downloads

Download (889KB)