Author(s):

Pratik Pusadkar and Zenu Jha

Abstract: In anther culture, in vitro regeneration of green plants is not more than 5-10 percent in indica rice cultivars, which limits the application of anther culture worldwide. To address this problem, in-vitro colchicine treatment was employed on callus obtained from anther culture of indica rice. S x R cross was taken for colchicine treatment after callus induction from anther culture treatment of 100 and 500 mg/l of colchicine was given for 48 and 72 hours and then after shifted to regenerative media. The experiment was performed along with control (without colchicine treatment) in which green callus percentage and overall plant regeneration percentage increases by 1 to 2.5 fold in treated calluses compared to control. The treatment containing 100 mg/l of colchicine in regeneration media followed by 48 hrs of incubation has given highest green callusing percentage of 23.13 % as control having only 9.16 % of green calli induction in S x R cross. The addition of colchicine had no detrimental effects on the different anther culture efficiency parameters. The highest numbers of green calluses were achieved in cross (SX R) 23 % while the lowest plant regeneration obtained in the cross (SRP) 3.1 %. The whole process up to rooting required seven week time plants obtained are transferred to hydroponics in Yoshida medium for primary hardening for about 20-25 days in glasshouse and subsequently secondary hardening done in field. This work is an attempt for standardization of regeneration media along with addition colchicine for increasing double haploid generation in Rice.

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