Author(s):

Namrata Parikh, Bhavik Chauhan and Dr. RC Mashru

Abstract: A new simple, precise, accurate and validated isocratic RP-HPLC method was developed for simultaneous determination of Gallic acid and Oleanolic acid in developed Polyherbal tablet formulation. The chromatographic condition for separation of these two phytoconstituents was C18 column diameter (4.6 x 250mm, 5μ), rheodyne manual injector with capacity of 20μL. The mobile phase was composed of mixture of 0.1% Ortho Phosphoric acid and Methanol (5:95), flow rate is 1 ml/min and detection was carried out at 222 nm. The run time was 15 min. Gallic acid was separated at 2.8 min and Oleanolic acid at 9.9 min, Resolution between these peaks was good. Validation of this developed method has been performed to demonstrate Precision, Linearity, Accuracy, Robustness, LOD and LOQ. Applicability of this validated method was carried out in lab scaled formulated dosage form and determine % Assay of both these phytoconstituents. Such type of chromatographic fingerprint analysis may useful tool for quantification of herbal and polyherbal formulation in future.

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