Author(s):

Gopala Krishna Chinnaboina, AMS Sudhakar Babu, Rajesh Verma, Pankaj Sharma and Birendra Shrivastava

Abstract: Diabetic nephropathy is a long-term complication of diabetic mellitus. Many experimental evidences suggest that persistent hyperglycemia generates intracellular reactive oxygen species (ROS), which is involved of free radicals in the pathogenesis of diabetes and more importantly in the development of diabetic complications. The objective of the present investigation was to evaluate the antidiabetic activity and protective effect of diabetic-induced nephropathy of ethanolic extract of Rhizophora mucronata flowers (ERMF) on streptozotocin (STZ) generated oxidative stress induced diabetic nephropathy in rats. Forty Wistar rats were indiscriminately distributed into normal control, diabetic untreated and diabetic treated with ERMF (250 and 500 mg/kg per body weight). Experimental diabetic nephropathy was induced by a single intraperitoneal (i.p) administration of STZ 60 mg/kg body weight (b.w.). The body weight, plasma glucose, creatinine, urea, uric acid and total protein along with oxidative stress parameters were examined in different groups of experimental animals. Histopathological examination was also performed for observing cellular changes on kidney tissues. Elevated level of blood sugar and urea whereas lower uric acid, creatinine, and total protein were detected in the STZ-diabetic rats, which were significantly attenuated after ERMF (250 and 500 mg/kg per body weight) administration, dose-dependently. Even various oxidative stress parameters were also drastically changed in a STZ-diabetic rats which were improved by ERMF (250 and 500 mg/kg per body weight). Histopathological study of kidney was supported the potential protective effect of ERMF (250 and 500 mg/kg per body weight). The activity elicited by the extract might be due to its ability to activate antioxidant enzymes. The findings suggest the potential use of the ERMF (250 and 500 mg/kg per body weight) as a novel therapeutically useful nephroprotective, agent.

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