Author(s):

KA Pagare, Dr. GG Khot and Dr. PB Mohite

Abstract: The single isolate of Bacillus thuringiensis was obtained from the twenty soil samples which were collected from Shahuwadi and Kagal tehsils of Kolhapur district of Western Ghat Zone of Maharashtra. The isolate was futher subjected for morphological and biochemical characterization. The isolate was having circular colonies, rough in nature, wavy margin, the colour of colony was creamy white having diameter 3mm. The isolate was subjected to the biochemical characterization showed positive reaction for catalase, starch hydrolysis, casein hydrolysis, nitrate reduction, voges- proskauer reaction, oxidase test and negative reaction for acid and gas, arginine dihydro lysis and esterase activity. The isolate was gram positive and motile. The cells were found to be producing endospores. The isolate possessed Bipyramidal shaped crystals when observed under microscope. The use of soil bacterium Bacillus thuringiensis has became immense potential for use of biopesticide. So there is currently need to isolate and identify Bacillus thuringiensis strain.

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