Background: Chassalia curviflora (Wall.) Thwaites occurs throughout the India. It is mainly used in folklore practise. It is widely used in diseased conditions like jaundice, malaria, coughs, wounds, and ulcers. Preliminary phytochemistry of leaves, stem, and flowers has already done and revealed the presence alkaloid, carbohydrate, saponins, phyto-steroids, triterpenoids, phenolic compounds, flavonoids, fixed oils and fats.

Aim: But so far the pharmacognostic standardization of root has not been reported for its proper identification. Hence the present study was carried out for the establishment of Pharmacognostical and phytochemical standardization of root.

Methods: Macroscopy, powder analysis (Organoleptic and powder microscopy), physiochemical properties like ash values, extractive values and HPTLC Profile of whole plant was done.

Results and Conclusion: Microscopy shows normal root structure with outer 5-7 layered cork, inner cortex, xylem and phloem. Medullary rays are uni to biseriate. Starch grains are present. HPTLC finger print suggested the presence of maximum number of peaks and maximum total area obtained in cold water extract. Qualitative phytochemical analysis showed the presence of alkaloids, flavanoids, tannins, saponins, steroids and glycosides. Maximum phytoconstituents was obtained for ethanolic extract.