Author(s):

P Boominathan, CV Chittibabu, C Sivaraj, K Saraswathi and B Nivetha

Abstract: Decalepis hamiltonii Wight & Arn. belongs to the family Asclepiadaceae. Its root tubers are utilized in tribal, traditional Indian and Chinese medicine for treatment of a wide range of ailments including digestive system, lungs and circulatory system. The aim of the present study was to evaluate the antioxidant and antibacterial activities of ethanol root tuber extract of D. hamiltonii and to identify the bioactive compounds by GC-MS analysis. Antioxidant activities such as DPPHË™ radical, superoxide radical (O₂^●-), hydroxyl radical (OH^●), ABTS^·+ radical cation, Fe³⁺ reducing power and phosphomolybdenum reduction assays were carried out. The maximum DPPHË™ radical scavenging activity of ethanol root tuber extract was 83.99±0.23% at 120 µg/mL concentration and the IC₅₀ was 40.32 µg/mL concentration. The maximum superoxide radical scavenging activity of ethanol root tuber extract was 73.73±0.11% at 120 µg/mL concentration and the IC₅₀ was 20.43 µg/mL concentration. The maximum OH^● radical scavenging activity was 66.54±0.48% at 120 µg/mL concentration and the IC₅₀ was 92.57 µg/mL concentration. The maximum ABTS^●+ radical cation scavenging activity of ethanol root tuber extract was 69.10±0.28% at 30 µg/mL concentration and the IC₅₀ was 24.35 µg/mL concentration. The maximum of Mo⁶⁺ reduction and Fe³⁺ reduction were 90.59±0.43% and 69.77±0.46% at 120 µg/mL concentration and the RC₅₀ values were 20.50 µg/mL and 21.15 µg/mL concentration. The antibacterial activity showed maximum zone of inhibition of 22 mm for Micrococcus luteus and Escherichia coli at 625 µg/mL concentration. GC-MS analysis of ethanol root tuber extract of D. Hamiltonii was carried out and antibacterial compound thujopsene-(12) and antioxidant compounds flavone and quinoxaline, 2-isopropyl-3-phenyl-4-oxide were identified.

Pages: 1493-1501  |  1122 Views  344 Downloads

Download (819KB)